| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.081503 |
| Chromosome: | chromosome 9 |
| Location: | 2498971 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g391050 | CYA9 | Adenylate cyclase; (1 of 22) PTHR11017//PTHR11017:SF145 - LEUCINE-RICH REPEAT-CONTAINING PROTEIN // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCCCTATTGGCTGTACACGGTGGAGGGCT |
| Internal bar code: | GTGGAAAGACGGCGAGCAGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 817 |
| LEAP-Seq percent confirming: | 95.0077 |
| LEAP-Seq n confirming: | 7993 |
| LEAP-Seq n nonconfirming: | 420 |
| LEAP-Seq n unique pos: | 37 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCTCCTCACCACCCTCTCT |
| Suggested primer 2: | ACTTGGGTCGAAAGACATCG |