| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.081526 |
| Chromosome: | chromosome 12 |
| Location: | 6335640 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g537350 | NZP1 | Zygote-specific hydroxyproline-rich glycoprotein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGTGCTATGTACTTACCTTTGTCGCCACC |
| Internal bar code: | GTAGTTTCCGTCGCGAAGCACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 736 |
| LEAP-Seq percent confirming: | 99.7462 |
| LEAP-Seq n confirming: | 9431 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGGAAGTTTTCTTGTGGGG |
| Suggested primer 2: | AGTTTGGTCTGCAGGGTCAC |