| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.081528 |
| Chromosome: | chromosome 12 |
| Location: | 623410 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g492350 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGTTCTATGATGTGCTCTGCATCAGCAA |
| Internal bar code: | ACGCTACCCATACGCCTCCCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 986 |
| LEAP-Seq percent confirming: | 99.6802 |
| LEAP-Seq n confirming: | 1870 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATTACCAACACTCGCCCTT |
| Suggested primer 2: | CACAGGAGCTGTATGCTGGA |