Insertion junction: LMJ.RY0402.081531_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.081531
Chromosome:chromosome 3
Location:4889593
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g179450 SRR7 (1 of 1) K09624 - protease, serine, 12 (neurotrypsin, motopsin) [EC:3.4.21.-] (PRSS12); Scavenger receptor cysteine rich (SRCR) protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGAGCACACGGTTGCGTTGTGACTAGAAA
Internal bar code:CCGGCAAGGGATAAGGCGAGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:1186
LEAP-Seq percent confirming:99.4778
LEAP-Seq n confirming:762
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:TTCATAGACGGCACTGCTTG
Suggested primer 2:CCCCACTGTCCCCTGTAGTA