Insertion junction: LMJ.RY0402.081531_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.081531
Chromosome:chromosome 3
Location:4889596
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g179450 SRR7 (1 of 1) K09624 - protease, serine, 12 (neurotrypsin, motopsin) [EC:3.4.21.-] (PRSS12); Scavenger receptor cysteine rich (SRCR) protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGGCCTGACTGCCCCGTGACTGGCCGGCCG
Internal bar code:TGAATAAATCCGTCTCGGCCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:343
LEAP-Seq percent confirming:98.9042
LEAP-Seq n confirming:2076
LEAP-Seq n nonconfirming:23
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:TTCATAGACGGCACTGCTTG
Suggested primer 2:CCCCACTGTCCCCTGTAGTA