| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.081556 |
| Chromosome: | chromosome 12 |
| Location: | 6959971 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g560250 | (1 of 5) PF04884 - Vitamin B6 photo-protection and homoeostasis (DUF647) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCACGGCAGAATCAGGCCTCGCGTGGTTG |
| Internal bar code: | CTGTGTTCACACGGGTCCGTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 414 |
| LEAP-Seq percent confirming: | 99.839 |
| LEAP-Seq n confirming: | 620 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTATCTGCACGGGTTCGAGT |
| Suggested primer 2: | CGTTTACGAGAGGCTTCAGG |