Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.081569 |
Chromosome: | chromosome_1 |
Location: | 4007474 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre01.g025983 | antisense | 5'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CCACTGCGACTTGTTGTTTCTGGGACACCC |
Internal bar code: | CGTCGGATGCGGCGCACCAGCG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 996 |
LEAP-Seq percent confirming: | 99.7046 |
LEAP-Seq n confirming: | 675 |
LEAP-Seq n nonconfirming: | 2 |
LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ATGTAGCATCCAGACCAGGG |
Suggested primer 2: | AGGGGTCGCTAACTATGGGT |