Insertion junction: LMJ.RY0402.081573_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.081573
Chromosome:chromosome 7
Location:760269
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre07.g317908 (1 of 1) K04485 - DNA repair protein RadA/Sms (sms, radA) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAGGGCGGCGCCAGGGGTGCGGCTGTGGCG
Internal bar code:TTAGCCATGTGAACGGTCGGAA

Confirmation - LEAP-Seq

LEAP-Seq distance:439
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:12
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:GTGTTGACACGGGACTGATG
Suggested primer 2:CAGGTACACGGTGTTGATGG