| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.081603 |
| Chromosome: | chromosome 8 |
| Location: | 4099671 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g379600 | (1 of 1) IPR001245//IPR002290//IPR011009 - Serine-threonine/tyrosine-protein kinase catalytic domain // Serine/threonine/dual specificity protein kinase, catalytic domain // Protein kinase-like domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCGACTGGAGGCCGAAGGGCACGGCTGT |
| Internal bar code: | TACTAAGCGGCGCATTTCGTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 145 |
| LEAP-Seq percent confirming: | 55.2901 |
| LEAP-Seq n confirming: | 1134 |
| LEAP-Seq n nonconfirming: | 917 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGCAGCACCTACAACAAGG |
| Suggested primer 2: | CCACGAAATGGAACACCAGT |