| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.081617 |
| Chromosome: | chromosome 14 |
| Location: | 3748923 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g632176 | (1 of 1) PTHR10641:SF660 - DNA-BINDING PROTEIN REB1-RELATED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCCGCCACCCCTCACCCCCTCCAGGTAC |
| Internal bar code: | AAGAGGGGGGAGGCGGACTTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 50 |
| LEAP-Seq percent confirming: | 92.7711 |
| LEAP-Seq n confirming: | 77 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCAGTGCAAGGAGAAGTG |
| Suggested primer 2: | TGTTGGCGTGTCTTAGCTTG |