Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.081645 |
Chromosome: | chromosome_9 |
Location: | 162598 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre09.g386736 | FAP44 | Flagellar Associated Protein | sense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TTCGAACGGACGCGCCAGCGGCCGACGCTG |
Internal bar code: | CTCAGTGGTCCTGTGACGGGCA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 322 |
LEAP-Seq percent confirming: | 98.941 |
LEAP-Seq n confirming: | 654 |
LEAP-Seq n nonconfirming: | 7 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GAAGGCATAATGGCTGGTGT |
Suggested primer 2: | GTCCTCCGCTTGTGAGTCAG |