Insertion junction: LMJ.RY0402.081648_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.081648
Chromosome:chromosome 13
Location:893445
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre13.g567650 (1 of 1) 3.4.14.2 - Dipeptidyl-peptidase II / Quiescent cell proline dipeptidase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACATCTTTCATTCGCGCACACACTTTACTC
Internal bar code:TACGCCCCCCGAATTAGCGAGA

Confirmation - LEAP-Seq

LEAP-Seq distance:240
LEAP-Seq percent confirming:99.7838
LEAP-Seq n confirming:5078
LEAP-Seq n nonconfirming:11
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:AGAAAGACCAGCGGCATCTA
Suggested primer 2:AATGTTACGGGGCTCATCTG