| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.081700 |
| Chromosome: | chromosome 2 |
| Location: | 8274074 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g141326 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGAGGGCTGCGCGAAGACCCGCCACAGCG |
| Internal bar code: | TGATACAGTTTGGGCGGGGAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 175 |
| LEAP-Seq percent confirming: | 67.7355 |
| LEAP-Seq n confirming: | 338 |
| LEAP-Seq n nonconfirming: | 161 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGACCCACGAACTTCACCAC |
| Suggested primer 2: | AGTTTGCCCGTAATCTGGTG |