| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.081702 |
| Chromosome: | chromosome 8 |
| Location: | 1615552 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g365103 | FAP319 | Coiled-Coil Flagellar Associated Protein 319 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGACCCTGCTGGCTGGGAAACGGTGACAAC |
| Internal bar code: | CCAGCTAGGCGGGTTCTGGAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 43 |
| LEAP-Seq percent confirming: | 82.0896 |
| LEAP-Seq n confirming: | 55 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATTTGACGCACAGCCTAGA |
| Suggested primer 2: | GACGCTGCCTCTGTACTTCC |