Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.081705 |
Chromosome: | chromosome 3 |
Location: | 7776128 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre03.g203250 | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTCCCCATGTTTCTCCTAGCGCGGGTGAG |
Internal bar code: | TCAATTCGAGAGTATTCCCCGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 64 |
LEAP-Seq percent confirming: | 98.7526 |
LEAP-Seq n confirming: | 475 |
LEAP-Seq n nonconfirming: | 6 |
LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CTTCCGGGGTAAGGTAGGAG |
Suggested primer 2: | TGCTGGTGTTTCTGACGAAG |