| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.081712 |
| Chromosome: | chromosome 12 |
| Location: | 1725398 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g512750 | TEF20 | Notchless-like protein; (1 of 1) PTHR22847//PTHR22847:SF50 - WD40 REPEAT PROTEIN // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACTTCGCGTCGCGGAAACCGCTTTCCGTG |
| Internal bar code: | GGATGCACACTATCAACTCAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 563 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 80 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGTCATCAAGCAAGTCGTC |
| Suggested primer 2: | GTGAGCGTGGGAATGGTAGT |