| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.081720 |
| Chromosome: | chromosome 6 |
| Location: | 2010967 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g264200 | SDH2 | (1 of 1) K00235 - succinate dehydrogenase (ubiquinone) iron-sulfur subunit (SDHB, SDH2); Iron-sulfur subunit of mitochondrial succinate dehydrogenase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATGAGCGTTACAGCTCTAATGTCCGCCTT |
| Internal bar code: | TGGTACTGCGTTGTCACGGTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1013 |
| LEAP-Seq percent confirming: | 99.7047 |
| LEAP-Seq n confirming: | 4052 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAAGTGTGGCTTGAGTGGT |
| Suggested primer 2: | GTGTAAGGTGTGCGTGGATG |