| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.081739 |
| Chromosome: | chromosome 10 |
| Location: | 2943351 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g440500 | (1 of 5) PF12738 - twin BRCT domain (PTCB-BRCT) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGGCTTCAGATGATGTGGACATGTGTGAG |
| Internal bar code: | CCGATAGAGCGCGGTCAACGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 808 |
| LEAP-Seq percent confirming: | 99.6531 |
| LEAP-Seq n confirming: | 2011 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGCAAGAAGTTGTGGCTTG |
| Suggested primer 2: | ACGAAATGGTAGAGGCGATG |