| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.081741 |
| Chromosome: | chromosome 3 |
| Location: | 3410747 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g166950 | PGM5 | (1 of 1) K01834 - 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (PGAM, gpmA); Phosphoglycerate mutase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGCAAGCGGCGGGTCGGACAGCGCTAACG |
| Internal bar code: | ACGTGATCAAGGGTTCGAATGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 448 |
| LEAP-Seq percent confirming: | 99.5227 |
| LEAP-Seq n confirming: | 417 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACATGACCCGAGGACAGTTC |
| Suggested primer 2: | GATGACGGTAGGTGCTGGTT |