| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.081746 |
| Chromosome: | chromosome_13 |
| Location: | 233254 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre13.g563700 | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GAGCGGGCGGGGGCGTGTGTGTGTGTGTGT |
| Internal bar code: | CCCTCGGGCCCATAGTGCGTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 186 |
| LEAP-Seq percent confirming: | 43.5463 |
| LEAP-Seq n confirming: | 5037 |
| LEAP-Seq n nonconfirming: | 6530 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAGCCCAAACTAAACCAAA |
| Suggested primer 2: | CACACGCACATGATTCCTTC |