Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.081752 |
Chromosome: | chromosome_11 |
Location: | 1698102 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre11.g467781 | FAP151 | ABC transporter-like and Flagellar Associated Protein | antisense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GTACCTGTGGGGGTGGATGGGCGAGGCGGG |
Internal bar code: | TCATGGGTGGCATTCAATGTCC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 410 |
LEAP-Seq percent confirming: | 99.7368 |
LEAP-Seq n confirming: | 379 |
LEAP-Seq n nonconfirming: | 1 |
LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GAGGTGGGGTTCACACTCAT |
Suggested primer 2: | GACACGCTCCTCCTGAAGAC |