Insertion junction: LMJ.RY0402.081781_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.081781
Chromosome:chromosome 1
Location:4410506
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre01.g029900 (1 of 3) 3.6.3.17 - Monosaccharide-transporting ATPase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCGACCCGCGGGCTGTTGCATCTGCATCG
Internal bar code:TCGCTCGAACACGGGGCGCGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:335
LEAP-Seq percent confirming:99.6869
LEAP-Seq n confirming:1592
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CTAGTCTTGAGCGCGGGTAG
Suggested primer 2:CATCGATGTGTCATTCCTCG