| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.081782 |
| Chromosome: | chromosome 16 |
| Location: | 1163715 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g650500 | (1 of 1) K06195 - ApaG protein (apaG) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCACTTGCGTTTGTCGCTTGTGCCTGCGCT |
| Internal bar code: | GTGCCACTGGGTACATGGCTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 593 |
| LEAP-Seq percent confirming: | 99.864 |
| LEAP-Seq n confirming: | 1469 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGTGCTGCTTTGTCAGTCG |
| Suggested primer 2: | CCCAAAACTCTTCAGCTTGC |