Insertion junction: LMJ.RY0402.081794_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.081794
Chromosome:chromosome 9
Location:4713579
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre09.g397031 (1 of 6) 5.3.99.5 - Thromboxane-A synthase / Thromboxane synthetase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTCGATACCAAATGTTAGACGCTATCTAT
Internal bar code:GATGGTCGATTTCGCCCCGCAT

Confirmation - LEAP-Seq

LEAP-Seq distance:766
LEAP-Seq percent confirming:97.8243
LEAP-Seq n confirming:6025
LEAP-Seq n nonconfirming:134
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CTGGTCTGCTGATGTGTGCT
Suggested primer 2:GTGGCGGTACCAATGAGACT