| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.081833 |
| Chromosome: | chromosome 16 |
| Location: | 5736460 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g678750 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCAGCTCGTTCGCGAGCGCTGCTGTCCCT |
| Internal bar code: | GCCATCCGGTTTAGGTGAAGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 283 |
| LEAP-Seq percent confirming: | 81.3387 |
| LEAP-Seq n confirming: | 401 |
| LEAP-Seq n nonconfirming: | 92 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCGTGTTACTGCTAGGCAC |
| Suggested primer 2: | ACATCCTAAACGCGGCATAC |