| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.081838 |
| Chromosome: | chromosome 15 |
| Location: | 663643 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g637750 | (1 of 7) IPR000104//IPR020683 - Antifreeze protein, type I // Ankyrin repeat-containing domain | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCAGCTATATCGTGTATCATATATGAAT |
| Internal bar code: | GGTTGTGACTAAGACGGGGCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 476 |
| LEAP-Seq percent confirming: | 98.7978 |
| LEAP-Seq n confirming: | 904 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTCGCCAAGTAGTAGTGG |
| Suggested primer 2: | CCATGGGATGGTGTGTATCA |