Insertion junction: LMJ.RY0402.081874_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.081874
Chromosome:chromosome 5
Location:1403249
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre05.g244250 DHC6 Axonemal Dynein Heavy Chain 6; (1 of 3) PTHR10676//PTHR10676:SF242 - DYNEIN HEAVY CHAIN FAMILY PROTEIN // DYNEIN HEAVY CHAIN 3, AXONEMAL CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGTGCGTGGTCTTCAACTGCTCCGACGGCC
Internal bar code:CAGGAGGGGTGATGTGTCCGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:352
LEAP-Seq percent confirming:81.749
LEAP-Seq n confirming:1075
LEAP-Seq n nonconfirming:240
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:ATCAACTGGGTGACCTCCTG
Suggested primer 2:CTGAGGTAGGGAACTGCTGC