Insertion junction: LMJ.RY0402.081878_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.081878
Chromosome:chromosome 9
Location:4010807
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre09.g392951 3'UTR_intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCACCGCCGCGGCCGCGACTCCCCAACATC
Internal bar code:AAGTAATGAGTGGCAATCGCGC

Confirmation - LEAP-Seq

LEAP-Seq distance:507
LEAP-Seq percent confirming:98.5907
LEAP-Seq n confirming:1679
LEAP-Seq n nonconfirming:24
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:ACCCGTCTGACAACAAGGAC
Suggested primer 2:CCTCACCCTAGCTTGTCAGC