| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.081894 |
| Chromosome: | chromosome 13 |
| Location: | 3940801 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g590700 | (1 of 4) PTHR16255//PTHR16255:SF4 - UNCHARACTERIZED // SPORULATION PROTEIN RMD8 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCAAGGGAATCAGAACGAATACGCGAATC |
| Internal bar code: | TTCCTGCACCTTTAGGCGTATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 404 |
| LEAP-Seq percent confirming: | 99.1254 |
| LEAP-Seq n confirming: | 2380 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTACCGCAATACTCACCCGT |
| Suggested primer 2: | GACCTGGTCGTAAGGTCCAA |