| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.081901 |
| Chromosome: | chromosome 13 |
| Location: | 798491 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g567075 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACACACGCACGCTCGGATAAATAGTCGGAC |
| Internal bar code: | CCCGCACGCTGAACGCGGGAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 155 |
| LEAP-Seq percent confirming: | 68.8445 |
| LEAP-Seq n confirming: | 1275 |
| LEAP-Seq n nonconfirming: | 577 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAGGCAGGCAAGCGATAAG |
| Suggested primer 2: | GATTGCTTTGTCGTACGGGT |