| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.081918 |
| Chromosome: | chromosome 3 |
| Location: | 4836306 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g178950 | HEL17 | (1 of 1) PTHR13710//PTHR13710:SF70 - DNA HELICASE RECQ FAMILY MEMBER // BLOOM SYNDROME PROTEIN; DEAD/DEAH box helicase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGGTGGTGGTGTGCCCGCTGCTGTCGCTG |
| Internal bar code: | GCCTGGATAGGAACGGGCGCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 306 |
| LEAP-Seq percent confirming: | 99.2331 |
| LEAP-Seq n confirming: | 647 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCAGTTGGCACTCCAGTA |
| Suggested primer 2: | CTGGTTGGATAGGGGCTACA |