| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.081928 |
| Chromosome: | chromosome 9 |
| Location: | 4840872 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g397549 | (1 of 1) PF06972 - Protein of unknown function (DUF1296) (DUF1296) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCAGCAAACGCCGTCCGTTGAGCGTCGTG |
| Internal bar code: | ATGGTGAGGAGGTGGTGCATCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 84 |
| LEAP-Seq percent confirming: | 93.6047 |
| LEAP-Seq n confirming: | 161 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACATTTCCATCCCGCTCTA |
| Suggested primer 2: | GCTCCCCGATGATGTCTAAA |