| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.082006 |
| Chromosome: | chromosome 7 |
| Location: | 1230434 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g321350 | (1 of 2) 3.1.3.1 - Alkaline phosphatase / Phosphomonoesterase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCATGGTGCCCCCCCCGTGTGGGGGCAGCG |
| Internal bar code: | GGTTCATGTGTGTCTAGGAGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 575 |
| LEAP-Seq percent confirming: | 99.397 |
| LEAP-Seq n confirming: | 2967 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAAGTAGACAGGGGCTCAG |
| Suggested primer 2: | CACACAACACAGCACCACAG |