| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.082023 |
| Chromosome: | chromosome 2 |
| Location: | 1365481 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g083065 | (1 of 2) 3.4.13.9 - Xaa-Pro dipeptidase / X-Pro dipeptidase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTCAGCGCCGCGCCTGCCACCGCTCCAT |
| Internal bar code: | CCGTCCAATCACGGAGGCGCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 67 |
| LEAP-Seq percent confirming: | 99.2308 |
| LEAP-Seq n confirming: | 516 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGTCTTCTTTCCCACTGC |
| Suggested primer 2: | AGCCAAGCGAAGAACACAGT |