| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.082027 |
| Chromosome: | chromosome 1 |
| Location: | 3065815 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g018950 | DHS1 | Deoxyhypusine synthase; (1 of 2) 2.5.1.46 - Deoxyhypusine synthase / Spermidine dehydrogenase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGTGTGTTACACCGCCCCGCGCAGCCGG |
| Internal bar code: | CGTCGGAAAAACTAGGGGAGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 225 |
| LEAP-Seq percent confirming: | 96.131 |
| LEAP-Seq n confirming: | 323 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAGGATTCGAACCTATGCG |
| Suggested primer 2: | GTGCGTGTACACTCGTGCTT |