| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.082030 |
| Chromosome: | chromosome 2 |
| Location: | 3807638 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g095550 | (1 of 1) PTHR14187//PTHR14187:SF51//PTHR22908 - ALPHA KINASE/ELONGATION FACTOR 2 KINASE // SUBFAMILY NOT NAMED // MIDASIN-RELATED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTTGCCGCTGGTGCTGCTGGGGGCCAACC |
| Internal bar code: | CGCTTCGATCGGGTGAGGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 475 |
| LEAP-Seq percent confirming: | 99.1477 |
| LEAP-Seq n confirming: | 698 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTAGTCAAGTCGGTGGGAA |
| Suggested primer 2: | CTAGCTGCTGTGCTGTCAGG |