| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.082081 |
| Chromosome: | chromosome 12 |
| Location: | 6388674 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g537641 | LPT1,LAT1 | (1 of 1) K13519 - lysophospholipid acyltransferase (LPT1, ALE1); Lysolipid Acyltransferase 1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCTACTGTACCTGCCTGCGCCTTCACTT |
| Internal bar code: | CCACCACGGTGGCATTTTTCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 264 |
| LEAP-Seq percent confirming: | 88.8298 |
| LEAP-Seq n confirming: | 334 |
| LEAP-Seq n nonconfirming: | 42 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGGCGCCTTCTGCTACTTA |
| Suggested primer 2: | ACACACACCCTCCACTCCTC |