| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.082094 |
| Chromosome: | chromosome 5 |
| Location: | 3125661 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g239200 | FAP275 | Flagellar Associated Protein 275 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTCCAATCGTCCTGGCACATCGCCCGTAC |
| Internal bar code: | GTGTGGCTCGTCCACTTATGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 471 |
| LEAP-Seq percent confirming: | 99.3603 |
| LEAP-Seq n confirming: | 1398 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGAGGTTTCGTTTGTGTTT |
| Suggested primer 2: | GTACCTGCATGTGCCAACAC |