| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.082130 |
| Chromosome: | chromosome 9 |
| Location: | 6964061 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g410650 | HIS1 | (1 of 1) 2.4.2.17 - ATP phosphoribosyltransferase / Phosphoribosyl-ATP pyrophosphorylase; N-5-phosphoribosyl-ATP transferase | intron|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCGCCCCCAAGCGCGGGGCCGCGGCGCGG |
| Internal bar code: | TCAAATGACATGTTTCGCGCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1275 |
| LEAP-Seq percent confirming: | 99.8134 |
| LEAP-Seq n confirming: | 4280 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTTACCGTATCCGGCAGTG |
| Suggested primer 2: | CGGGTTGGTGTTTTGATAGC |