| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.082138 |
| Chromosome: | chromosome 12 |
| Location: | 3951851 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g516350 | COX10 | Mitochondrial cytochrome c oxidase assembly protein; (1 of 1) K02257 - protoheme IX farnesyltransferase (COX10) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCCTGGAGCCTGCCGTTACGCGCCGTTAT |
| Internal bar code: | ATCTGTGTCGGATGAGGAGTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 706 |
| LEAP-Seq percent confirming: | 99.2626 |
| LEAP-Seq n confirming: | 2423 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTAACACCATTACGGTGGGG |
| Suggested primer 2: | CTGGATTGCATGTAACGTGG |