Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.082141 |
Chromosome: | chromosome 2 |
Location: | 6945496 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre02.g120200 | (1 of 4) 4.1.1.39 - Ribulose-bisphosphate carboxylase / RuBP carboxylase | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGCATCCGTCAGCAAAAGTAACTCTGGCG |
Internal bar code: | GCAAAGTTAGCGCCCTCGGGGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 179 |
LEAP-Seq percent confirming: | 74.9498 |
LEAP-Seq n confirming: | 20920 |
LEAP-Seq n nonconfirming: | 6992 |
LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GTGCAACAATGTCTCTGCGT |
Suggested primer 2: | TGTCGCATTCATAATCCCAA |