| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.082166 |
| Chromosome: | chromosome 13 |
| Location: | 4465200 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g603450 | (1 of 4) PF14159 - CAAD domains of cyanobacterial aminoacyl-tRNA synthetase (CAAD) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCATGCGCCCATGCACTCACCGGCTCCGGG |
| Internal bar code: | GAGAGCGTGTGGTTAGAACGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 130 |
| LEAP-Seq percent confirming: | 35.442 |
| LEAP-Seq n confirming: | 437 |
| LEAP-Seq n nonconfirming: | 796 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACGTAGACCAGGTGTGTGG |
| Suggested primer 2: | GTCGGGTAAAGCGTACCAAA |