Insertion junction: LMJ.RY0402.082168_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.082168
Chromosome:chromosome 1
Location:7617614
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre01.g055000 (1 of 2) 3.6.1.17 - Bis(5'-nucleosyl)-tetraphosphatase (asymmetrical) / Dinucleosidetetraphosphatase (asymmetrical) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGTGCACTTAGGCGCTGCCGCTCCTTATGG
Internal bar code:CCTGTTGATAGGGCGAAGACTG

Confirmation - LEAP-Seq

LEAP-Seq distance:164
LEAP-Seq percent confirming:97.9424
LEAP-Seq n confirming:238
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CGGCACTCCATACGGTACTT
Suggested primer 2:AAATTGGCTGGTTCACTTGG