| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.082198 |
| Chromosome: | chromosome 7 |
| Location: | 1835136 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g325731 | (1 of 6) PF01925 - Sulfite exporter TauE/SafE (TauE) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTAGGAGTGGGATTCCCGCGACCATGCACC |
| Internal bar code: | GGGCGTCGCGCTCGGGCCATCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 402 |
| LEAP-Seq percent confirming: | 99.7647 |
| LEAP-Seq n confirming: | 848 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAGGCCTATTCGAGCAGTT |
| Suggested primer 2: | AGGTAAATTGGTGTGCGAGG |