| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.082227 |
| Chromosome: | chromosome 2 |
| Location: | 7204624 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g146150 | (1 of 4) PF05066 - HB1, ASXL, restriction endonuclease HTH domain (HARE-HTH) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCGCCGTTGATGACGGGCGCCGCTGCGGC |
| Internal bar code: | GCGCCTAGCACGGGAGCGTTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 107 |
| LEAP-Seq percent confirming: | 90.7767 |
| LEAP-Seq n confirming: | 187 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGCTTCTAGCAGCTTTGTT |
| Suggested primer 2: | GCAGGCAGATCCTTGCTAAC |