Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.082253 |
Chromosome: | chromosome_16 |
Location: | 3525370 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre16.g677382 | sense | 5'UTR|outside_mRNA |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CGCTAATTTGGCTCACACAAGGAGGCTCCA |
Internal bar code: | ATATAGTGCCGTATGTGGATCA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 715 |
LEAP-Seq percent confirming: | 98.4869 |
LEAP-Seq n confirming: | 716 |
LEAP-Seq n nonconfirming: | 11 |
LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GTCTACAGCTGAAGGGGCAG |
Suggested primer 2: | GCACATAGCTACAGGCACGA |