| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.082299 |
| Chromosome: | chromosome 17 |
| Location: | 4170842 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g729950 | SRX1,SRX2 | (1 of 2) 1.8.98.2 - Sulfiredoxin / Peroxiredoxin-(S-hydroxy-S-oxocysteine) reductase; Related to sulfiredoxin | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGGAACTACCGGTTGCGGCCATCCGCAG |
| Internal bar code: | ATTCCCCATCATGCGATCTGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 448 |
| LEAP-Seq percent confirming: | 99.6393 |
| LEAP-Seq n confirming: | 10496 |
| LEAP-Seq n nonconfirming: | 38 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGTTTGGTTTAGTCTGGGC |
| Suggested primer 2: | CGCACGAGAGTCATCAGGTA |