| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.082311 |
| Chromosome: | chromosome 16 |
| Location: | 5896513 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g677200 | (1 of 274) IPR020683 - Ankyrin repeat-containing domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCATGTAGATTTTGGCAAGGGAATTTTGTT |
| Internal bar code: | AGTTGTGCTACACTATTGCTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 275 |
| LEAP-Seq percent confirming: | 99.8035 |
| LEAP-Seq n confirming: | 508 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATGCAGACTCAGCAAACGG |
| Suggested primer 2: | CCACTCACGACATCCATCAG |