| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.082322 |
| Chromosome: | chromosome 6 |
| Location: | 6102402 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g289900 | HEN2 | (1 of 24) PTHR10641 - MYB-LIKE DNA-BINDING PROTEIN MYB; Hua Enhancer 2 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCCGAGGGAAAGAAGACGCTGAACGATTG |
| Internal bar code: | TGTTCCTCCAATTAGGCCCACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 100 |
| LEAP-Seq percent confirming: | 62.2995 |
| LEAP-Seq n confirming: | 233 |
| LEAP-Seq n nonconfirming: | 141 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAGTACCTATCCATGCGCC |
| Suggested primer 2: | CGCGACACATACATACCGTC |