| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.082341 |
| Chromosome: | chromosome 1 |
| Location: | 3372606 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g021450 | TPR4 | Tetratricopeptide-repeat protein 4; (1 of 1) PTHR23083//PTHR23083:SF443 - TETRATRICOPEPTIDE REPEAT PROTEIN, TPR // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCGCAGTGGTGGGCCCACGCAAGGACCG |
| Internal bar code: | GCGCAGTATACTGGCTTGGCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 470 |
| LEAP-Seq percent confirming: | 99.1453 |
| LEAP-Seq n confirming: | 2320 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTAACCCTCGCGACCAGTA |
| Suggested primer 2: | GCGTTACCTTATGCGAGAGC |